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学位論文 |
学位
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木暮, 憲道
概要:
Thesis or Dissertation<br />学位記番号：医博甲1625
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学位論文 |
学位
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本城, 裕章
概要:
Thesis or Dissertation<br />学位記番号：医博甲1628
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| 3.
学位論文 |
学位
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高橋, 研吾
概要:
Thesis or Dissertation<br />学位記番号：医博甲1587
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| 4.
学位論文 |
学位
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Tanaka, Yuko ; Sasaki, Atsushi ; Ishiuchi, Shogo ; Nakazato, Yoich
概要:
application/pdf<br />Thesis or Dissertation<br />To characterize the cellular density and proliferative activity of glia
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l fibrillary acidic protein (GFAP)-negative cells in pilocytic astrocytoma (PA), surgically excised tissues of PAs (n=37) and diffuse astrocytomas (DAs) (n=11) were examined morphologically and immunohistochemically using antibodies against GFAP, Olig2, Iba1 and Ki-67 (MIB-1). In PA, Olig2 immunoreactivity was significantly expressed in protoplasmic astrocytes in microcystic, loose areas and cells in oligodendroglioma-like areas. Iba1-positive, activated microglia/macrophages were also commonly observed in microcystic areas. In compact areas, a prominent reaction for GFAP was observed, but for Olig2 and Iba1 to a lesser degree. On semiquantitative analysis, the number of Olig2-positive cells was significantly higher in PAs (mean labeling index (LI) ± standard deviation (SD): 46.8 ± 15.4%) than in DAs (13.3 ± 7.8%) (P<0.001). Many Iba1-positive, microglia/macrophages were observed in PAs (19.9 ± 6.5%), similarly to DAs (20.9 ± 9.9%). Re-immunostaining of PA demonstrated that most Ki-67-positive, proliferating cells expressed Olig2, whereas GFAP or Iba1 expression in Ki-67-positive cells was less frequent (14.7 ± 13.7%, and 8.8 ± 13.6%) in a double immunostaining study. Conversely, the percentage of Olig2-positive, proliferating cells in total Olig2-positive cells (7.2 ± 3.9%) was higher than that of Iba1-positive, proliferating cells in total Iba1-positive cells (0.9 ± 0.6%). In conclusion, the present study found that PA consisted of numerous GFAP-negative cells, including Olig2-positive cells with high proliferation. Semiquantitative analysis of Olig2 immunohistochemistry in microcystic areas might therefore be useful for the differential diagnosis of PA and DA<br />学位記番号：医博甲1088
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| 5.
論文 |
論文
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Ikota, Hayato ; Kinjo, Sawako ; Yokoo, Hideaki ; Nakazato, Yoichi
概要:
application/pdf<br />Journal Article<br />We performed a systematic immunohistochemical study on 378 brain tumors using 37 antibodies and tissue microarray (TMA) technology. The aim of this study was to find new diagnostic
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biomarkers using antibodies established in our laboratory. Our TMA consisted of a grid of 1.5-mm cores that were extracted from individual donor blocks. Staining for each antibody was scored using a three-point system. We used hierarchical clustering analysis to interpret these data, which resulted in separation of all the brain tumors into seven groups. Although there were some exceptions, cases with the same histological diagnosis were generally grouped together. We then carried out statistical analyses to find the most useful antibodies for grouping of brain tumors. Ten antibodies [glial fibrillary acidic protein (GFAP), Olig2, vimentin, epithelial membrane antigen (EMA), cytokeratin (AE1/AE3), alpha-internexin, nestin, pinealocytes PP5, aquaporin-4 (AQP4) M13d and AQP4M13e] discriminated between astrocytomas and oligodendroglial tumors. Six antibodies [EMA, AE1/AE3, TUJ1, nestin, neurofilament protein-MH (NF-MH) and perivascular cells GP-1] showed significant differences between high-grade and low-grade gliomas. Our data have revealed new antibodies with potential diagnostic utility (Olig2, PP5, GP-1) and demonstrate that TMA technology is highly useful for evaluating newly established antibodies in brain-tumor research.<br />The original publication is available at www.springerlink.com
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| 6.
論文 |
論文
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Yamaguchi, Haruyasu ; Okamoto, Koichi ; Shooji, Mikio ; Morimatsu, Mitsunori ; Hirai, Shunsaku
概要:
application/pdf<br />Departmental Bulletin Paper<br />A woman with idiopathic hypoparathyroidism presented with proximal limb weakness, easy fatigability, hoarseness and elevated serum creatine kinase associated with hypocalcemia. As
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these findings disappeared after normalization of the serum calcium level with treatment, the diagnosis of hypocalcemic myopathy was established. Muscle biopsy showed type 2 fiber atrophy and. Immunohistochemical investigation revealed myoglobin loss from some muscle fibers, suggesting that hypocalcemia itself may produce myopathy associated with myoglobin loss without structural changes.
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学位論文 |
学位
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7. High membrane expression of CMTM6 in hepatocellular carcinoma is associated with tumor recurrence
村主, 遼
概要:
Thesis or Dissertation<br />学位記番号：医博甲1928
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| 8.
学位論文 |
学位
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8. High membrane expression of CMTM6 in hepatocellular carcinoma is associated with tumor recurrence
村主, 遼
概要:
Thesis or Dissertation<br />学位記番号：医博甲1928
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