群馬大学図書館 OPAC

概要: application/pdf<br />Departmental Bulletin Paper<br />The purpose of this study was to examine the effects of intravenous injection of\nurotensinII on the systemic blood pressure and heart rate, and to … examine whether the\nintraperitoneal administration of urotensinII evoked the stimulation of central neurons.\nUrotensin, originally isolated fish neuro-endocrine systems, is a peptide which consists of 12\namino acids. Lately human urotensinII was identified to be consisted from 11 amino acids.\nReceptors of urotensin have been found to highly distribute in the mammalian cadrdiovascular\nsystems. It has been reported that urotensin evoked contraction of thoracic artery, while the\ndilatation of blood vessels was alternatively induced. In this study, we examined the effects of\nperipheral injection of rat urotensinII consisted of 14 amino acids on the circulation and on the\nactivity of neurons in the brain. It was found that intravenous injection of rat urotensinII\nelicited dose-dependently decrease in blood pressure, and increase or decrease in heart rate.\nTo examine whether an intraperitoneal injection of rat urotensinII induced an excitation of\ncentral neurons, we used immunohistochemical method to study the expression of c-Fos\nprotein in neurons of the rat brain after intraperitoneal injection of rat urotensinII (test\nexperiment). In the control experiment rat was intraperitoneally injected saline solution\nwithout rat urotensinII. It was found that intraperitoneal injection of rat urotensinII induced\nexpression of c-Fos protein in several nuclei in the brain. These results suggested that rat\nurotensinII might exhibit physiological functions via central neuronal pathway as well as\nperipheral direct pathways. 続きを見る

概要: application/pdf<br />Departmental Bulletin Paper<br />Leptin has been recognized to be an important neuroendocrine signa … l in the\nregulation of food intake and energy balance. We aimed to survey central neurons that might be\nactivated after peripheral administration of leptin, by examining the distribution of neurons\nexpressing c-Fos protein. Leptin dissolved at a dose of 500 μg/kg in physiological saline was\nintraperitoneally injected in Wistar rats. One and a half hours after the injection, rats were\ntranscardially perfused with saline and fixed with fixatives. The brain was removed and\nsectioned at 40 μm in thickness. Every fourth section was treated with anti-c-Fos antiserum,\nand c-Fos protein was immunohistochemically stained using the avidin-biotin complex method.\nControl rats were injected with saline solution, and brain sections were processed similarly as\ndescribed above. It was found that leptin injected intraperitoneally induced the neuronal\nexpression of c-Fos protein in several nuclei throughout the brain. In the central nucleus\namygdala, ventromedial nucleus of hypothalamus, periaqueductal gray matter, lateral\nparabrachial nucleus, and the solitary tract nucleus, numbers of neurons expressing c-Fos\nprotein were much more in the test experiments than those in the control experiments.\nIntraperitoneally injected leptin was found to stimulate central neurons that may play some\nroles in the regulation of such as a food intake. 続きを見る

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