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論文
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新井, 治子 ; 廣町, 佐智子 ; 阿久澤, 由紀 ; 伊藤, まゆみ ; 伊藤, 善一
概要:
application/pdf<br />Departmental Bulletin Paper<br />The aim of this study was to evaluate the efficacy of umbilicus cleansing on the basis of the bacterial effects of Isodine sterilization. The subjects were 100G college students,
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divided into a cleansing and a non-cleansing group. The number and species of bacteria in the cleansing group and in the non-cleansing group were compared. The results showed that both the total number of bacteria and the number of anaerobic bacteria were significantly lower in the cleansing group than in the non-cleansing group. This suggested that umbilicus cleansing is an effective mean of eliminating bacteria which are unaffected by Isodine sterilization.
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論文
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小河原, はつ江 ; 金谷, 尚美 ; 高橋, 美紀子 ; 阿久澤, 由紀 ; 倉茂, 達徳 ; 土屋, 純
概要:
application/pdf<br />Departmental Bulletin Paper<br />Immunocytochemical measurements of lymphocyte blastogenesis in cell smears with antibromodeoxyuridine monoclonal antibodies were investigated. In this method,
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neither radioisotopes nor special apparatus except for a CO_2-incubator are necessary, and accurate values can be obtained without difficulty. Additionally, these values correlate closely with the results obtained by the tritiated thymidine method. Therefore, it was suggested that this method is clinically useful as a screening test for lymphocyte blastogenesis.
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論文
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阿久澤, 由紀 ; 三石, 俊美 ; 倉茂, 達徳
概要:
application/pdf<br />Departmental Bulletin Paper<br />Time-resolved fluorometry was used to detect the cytotoxic activity of lymphocytes. The target cells were labeled with Europium instead of ^<51>Cr in dextran sulfate-containing Europium
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solution. K562 cells and S180 cells were applicable to use as target cells, if they were labeled with Europium in optimal dextran sulfate concentration and appropriate labeling time. The results obtained showed that this method was able to apply to the measurement of cytotoxic activity of natural killer (NK) cell and of lymphokine-activated killer (LAK) cell.
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