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梶野, 円香 ; 斉藤, 誠子 ; 佐竹, 幸子 ; 倉茂, 達徳
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application/pdf<br />Departmental Bulletin Paper<br />A student exchange program between the medical technology departments of Gunma University and the University of Washington was begun as an experiment and should be given official
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recognition upon a final agreement between the two universities. For two wee
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小山, 洋 ; 野田, 広 ; 小林, 隆弘 ; 田中, 一雄 ; 三上, 紘一 ; 浜名, 康栄 ; 村上, 博和 ; 山口, 晴保 ; 佐藤, 久美子 ; 倉茂, 達徳 ; 鈴木, 庄亮
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application/pdf<br />Departmental Bulletin Paper
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阿久沢, 由紀 ; 倉茂, 達徳
概要:
application/pdf<br />Departmental Bulletin Paper<br />We studied in this paper on the effects of the operation on natural killer (NK) cells and lymphokine-activated killer (LAK) cells in the cancer patients. The cytotoxic
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activities of lymphocytes against both K562 cells and Daudi cells were significantly suppressed by the operation when those activities were determined at 2 weeks after operation and their activities recovered at 4 weeks after. However, lymphocytes collected at 2 weeks after operation and stimulated in vitro with IL2 showed the same activity against K562 cells as those of lymphocytes collected before operation but did not against Daudi cells. By the CD antigen analysis, in lymphocytes collected from the cancer patients, the number of CD16-positive-CD56-negative cells were not effected by the operation and significant increase was observed by the in vitro IL2 stimulation. By the contrast, CD16(+)-CD56(+) cells were markedly reduced in their number and were not increased by the in vitro IL2 stimulation, whereas the number of CD16(+)-CD56(+) cells increased after IL2 stimulation in the lymphocytes collected from normal human. These results suggest that the operation does not induce NK cell deficiency but dose LAK cell deficiency through the defect of differentiation of CD16(+)-CD56(-) NK cells to CD16(+)-CD56(+) LAK cells or of CD16(+)-56(+) progenitor cells to mature LAK cells.
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阿久沢, 由紀 ; 甕, その子 ; 関口, 三郎 ; 竹沢, 和男 ; 中村, 正江 ; 植原, 奈美子 ; 森, 初恵 ; 杉田, 光章 ; 新部, 万理子 ; 三石, 俊美 ; 倉茂, 達徳
概要:
application/pdf<br />Departmental Bulletin Paper<br />We studied in this paper the effects of pregnancy on nonspecific immunity of neutro-philes and specific immunity of T-lymphocytes. The number of neutrophiles increased and that of
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lymphocytes conversely decreased during pregnancy. The phagocytic activity of neutrophiles derived from pregnant woman was enhanced by the autoserum as same as that derived from normal non-pregnant woman. By the contrast, the lymphocytes derived from pregnant woman showed low blastogenic respons iveness to Con A. The sera from pregnant women remarkably suppressed the blastogenic activity of lymphocytes prepared from both normal and pregnant women. Moreover, the lymphocytes from pregnant woman was much more sensitive to suppression by the serum fact or as compared with the lymphocytes from non-pregnant woman. The suppression by the serum was not due to cortizole that increased in the serum during pregnancy. These results suggest that specific cellular immunity of T-lymphocytes was suppressed to avoid fetal rejection and that nonspecific immunity of neutrophiles was enhanced to escape microbial infections during pregnancy.
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小河原, はつ江 ; 金谷, 尚美 ; 高橋, 美紀子 ; 阿久澤, 由紀 ; 倉茂, 達徳 ; 土屋, 純
概要:
application/pdf<br />Departmental Bulletin Paper<br />Immunocytochemical measurements of lymphocyte blastogenesis in cell smears with antibromodeoxyuridine monoclonal antibodies were investigated. In this method,
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neither radioisotopes nor special apparatus except for a CO_2-incubator are necessary, and accurate values can be obtained without difficulty. Additionally, these values correlate closely with the results obtained by the tritiated thymidine method. Therefore, it was suggested that this method is clinically useful as a screening test for lymphocyte blastogenesis.
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阿久澤, 由紀 ; 三石, 俊美 ; 倉茂, 達徳
概要:
application/pdf<br />Departmental Bulletin Paper<br />Time-resolved fluorometry was used to detect the cytotoxic activity of lymphocytes. The target cells were labeled with Europium instead of ^<51>Cr in dextran sulfate-containing Europium
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solution. K562 cells and S180 cells were applicable to use as target cells, if they were labeled with Europium in optimal dextran sulfate concentration and appropriate labeling time. The results obtained showed that this method was able to apply to the measurement of cytotoxic activity of natural killer (NK) cell and of lymphokine-activated killer (LAK) cell.
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青柳, 英子 ; 阿久沢, 由紀 ; 瀬戸, 正子 ; 正田, 美智子 ; 倉茂, 達徳
概要:
application/pdf<br />Departmental Bulletin Paper<br />Using almost all students of our college, the efficacy of hand-washing was determined by the bacterial colony formation method on an agar plate. A medicated soap which contains
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trichlorocarbanilide, an antibacterial agent, showed no certain disinfection effect in all the cases who washed with the soap once for 10 to 60 sec and in about half the cases who washed 3 times with the soap for 30 sec in combination with water for 30 sec. Chlorhexidine (hibitane) is one of the most useful disinfectants for hand-washing. Satisfactory disinfection effect was observed in 90% of cases by washing with hibitane at a concentration of 0.1% for 20 sec, but at 0.02 and 0.05%, generally used concentrations, that of hibitane declined to 42 and 60%, respectively. Disinfection with a wet-cotton is a convenient method but its efficacy in our experiment was far less than that of handwashing with the disinfectant solution. We recommend from our results a wet-cotton with the mixed solution of 0.1% hibitane and 80% ethanol, that showed reliable disinfection effect in almost all cases.
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宮脇, 修一 ; 倉茂, 達徳 ; 中野, 稔 ; 三橋, 進
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application/pdf<br />Departmental Bulletin Paper<br />O_2 consumption method assaying cytotoxic activity of 6MP was compared with other 4 methods; dye exclusion method, colonyformation inhibition method, ^3H-thymidihe uptake
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inhibition method and growth inhibition method using L1210 mouse leukemic cells and 6MP. The most sensitive method for cytotoxicity assay was colony formation inhibition method on an agar plate but at least 10 days are necessary for determination. Dye exclusion method was the simplest but sensitivity was the lowest among 5 methods and 16 hours are required for the evaluation. O_2 consumption method was more sensitive and reliable than dye exclusion method. Moreover, O_2 consumption method was the simplest among 4 methods except dye exclusion method and the results could be obtained within 8 hour-treatment with 6MP. These results suggest that O_2 consumption method is an useful and convenient for evaluating drug sensitivity of leukemic cells.
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| 9.
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小河原, はつ江 ; 村上, 博和 ; 吉田, 寿春 ; 倉茂, 達徳 ; 佐藤, 久美子 ; 土屋, 純 ; 高桑, 正道 ; 小林, 紀夫 ; 前川, 正
概要:
application/pdf<br />Departmental Bulletin Paper
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