Proliferation and cell death of human glioblastoma cells after carbon-ion beam exposure: Morphologic and morphometric analyses

フォーマット:

学位論文

責任表示:

Oishi, Takuma ; Sasaki, Aatsushi ; Hamada, Nobuyuki ; Ishiuchi, Shogo ; Funayama, Tomoo ; Sakashita, Tetsuya ; Kobayashi, Yasuhiko ; Nakano, Takashi ; Nakazato, Yoichi

言語:

英語

出版情報:

群馬大学医学系研究科, 2008

著者名:

Oishi, Takuma

Sasaki, Aatsushi

Hamada, Nobuyuki

Ishiuchi, Shogo

Funayama, Tomoo

Sakashita, Tetsuya

Kobayashi, Yasuhiko

Nakano, Takashi

Nakazato, Yoichi

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バージョン:

AM

概要:

application/pdf<br />Thesis or Dissertation<br />Histological analyses of glioblastoma cells after carbon-ion exposure are still limited and ultrastructural characteristics have not been investigated in detail. Here we report th … e results of morphological and morphometric analyses of a human glioblastoma cell line, CGNH-89, after ionizing radiation to characterize the effect of a carbon-beam on glioblastoma cells. Using CGNH-89 cells exposed to 0–10 Gy of X-ray (140kVp) or carbon-ions (18.3 MeV/nucleon, LET = 108 keV/μm), we performed conventional histology and immunocytochemistry with MIB-1 antibody, transmission electron microscopy, and computer-assisted, nuclear size measurements. CGNH-89 cells with a G to A transition in codon 280 in exon 8 of the TP53 gene had nuclei with pleomorphism, marked nuclear atypia and brisk mitotic activity. After carbon-ion and X-ray exposure, living cells showed decreased cell number, nuclear condensation, increased atypical mitotic figures, and a tendency of cytoplasmic enlargement at the level of light microscopy. The deviation of the nuclear area size increased during 48 hours after irradiation, while the small cell fraction increased in 336 hours. In glioblastoma cells of the control, 5 Gy carbon-beam, and 10 Gy carbon-beam, and MIB-1 labeling index decreased in 24 hours (12%, 11%, 7%, respectively) but increased in 48 hours (10%, 20%, 21%, respectively). Ultrastructurally, cellular enlargement seemed to depend on vacuolation, swelling of mitochondria, and increase of cellular organelles, such as the cytoskeleton and secondary lysosome. We could not observe apoptotic bodies in the CGNH-89 cells under any conditions. We conclude that carbon-ion irradiation induced cell death and senescence in a glioblastoma cell line with mutant TP53. Our results indicated that the increase of large cells with enlarged and bizarre nuclei, swollen mitochondria, and secondary lysosome occurred in glioblastoma cells after carbon-beam exposure.<br />学位記番号：医博甲1096 続きを見る