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学位論文 |
学位
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Olia, Alex
概要:
Thesis or Dissertation<br />学位記番号：医博甲1797
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論文 |
論文
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Kaneko, Yuka ; Kaneko, Yoriaki ; Ohnishi, Hiroshi ; Tomizawa, Takeshi ; Okajo, Jun ; Saito, Yasuyuki ; Okuzawa, Chie ; Murata, Yoji ; Okazawa, Hideki ; Nojima, Yoshihisa ; Okamoto, Koichi ; Matozaki, Takashi
概要:
application/pdf<br />Journal Article<br />Background & Aims: SHPS-1 is a transmembrane protein that binds the protein ty
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rosine phosphatases \nSHP-1 and SHP-2 through its cytoplasmic region. It is highly expressed on the surface of CD11c+\ndendritic cells (DCs) and macrophages. We have recently shown that priming of CD4+T cells by DCs \nis markedly impaired in mice that express a mutant form of SHPS-1 lacking most of the cytoplasmic \nregion. We have now evaluated further the functions of CD4+T cells derived from SHPS-1 mutant mice. \nMethods: The expression of cell surface molecules on CD4+T cells was examined by flow cytometry. \nThe proliferation of CD4+T cells was measured by［3H］thymidine incorporation. Cytokine production \nby CD4+T cells was measured by ELISA. Results: SHPS-1 is expressed at low level on CD4+T \ncells of wild-type mice. The T cell receptor (TCR)-stimulated proliferation of CD4+T cells from \nSHPS-1 mutant mice was markedly decreased, whereas the TCR-stimulated production of IL-2 and \nIFN-γ by these cells was markedly increased, compared with those apparent with wild-type cells. \nDifferentiation of CD4+T cells from SHPS-1 mutant mice into Th1 cells was also impaired. Conclusions: Present results suggest that SHPS-1 is essential for proper regulation of CD4+T cell functions.
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