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論文
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GOUMA, Kanako ; SATO, Wakako ; INO, Yurie ; UMEMURA, Yukiko ; MOCHIZUKI, Chiharu ; TAKATSUKA, Kenji ; KIGURE, Yoshie ; TANAKA, Nagisa ; ITOH, Takashi ; YOKOTA, Akira
概要:
application/pdf<br />Departmental Bulletin Paper<br />Cellular polyamines extracted from reclassified or newly validated 47\nalphaproteobacteria, 46 betaproteobacteria, 96 gammaproteobacteria, 12 deltaproteobacteria\nand 10
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epsilonproteobacteria were analyzed by high-performance liquid chromatography.\nHomospermidine was widely distributed within the class Alphaproteobacteria, however,\nhomospermidine-dominant type, spermidine-dominant type and homospermidine/spermidinedominant\ntype were found and the three triamine profiles were genus-specific. The all genera\nbelonging to the class Betaproteobacteria, ubiquitously contained putrescine and 2-\nhydroxyputrescine. Triamines were absent in almost betaproteobacteria. Many genera,\nincluding psychrophilic species, of the class Gammaproteobacteria, contained putrescine and\nspermidine as the major polyaminenes. Diaminopropane and norspermidine were selectively\ndistributed in several genera of the class Gammaproteobacteria. Spermidine was the major\npolyamine in the classes Deltaproteobacteria and Epsilonproteobacteria. Spermine was found\nin some thermophiles within Betaproteobacteria, Deltaproteobacteria and\nEpsilonproteobacteria, suggesting that the occurrence of spermine correlate to their\nthermophily. Additional these polyamine catalogues serve for the classification of the phylum\nProteobacteria, as a chemotaxonomic marker.
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論文
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大塚, 恵美子 ; 浜名, 康栄 ; 新津, 勝
概要:
application/pdf<br />Departmental Bulletin Paper<br />マメ科8種,Cicer arietinum(ヒヨコマメ), Glycine max(アオダイズ,Glycine max)(クロダイズ
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),Lablab niger(クラカケマメ), Lens culinaris(レンズマメ),Phaseolus vulgaris(クロインゲン), Rudua aurea(リョクトウ),Rudua mungo(ケツルアズキ)の成熟種子(豆)より過塩素酸抽出したポリアミン画分を高性能液体クロマトグラフィーで分析した。さらに,イオン交換クロマトグラフィーにて濃縮・精製したポリアミン画分のガスクロマトグラフィー分析も行った。スペルミジンとスペルミンは主成分として,プトレスシンとホモスペルミジンは微量成分として,全8種に存在した。ジアミノプロパン,カダベリン,アグマテン,アミノプロピルホモスペルミジン,カナパルミンは4-7種の成熟種子中に検出された。アミノプロピルホモスペルミジンとカナパルミンは未熟種子では検出されず,成熟に伴って生成されることがわかった。発芽種子からのポリアミン画分の分析から,ジアミノプロパン生成の上昇(P. vulgaris, R.mungo),カダベリン生成の上昇(G.max, R. aurea)プトレスシンとカダベリン生成の上昇(C. arietinum, L. culinaris)が認められた。以上の結果をすでに分析済みのマメ科38種の成熟種子と発芽種子のポリアミン分析結果と比較した。
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論文
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Hamana, Koei ; Hamana, Hiroshi ; Shinozawa, Takao
概要:
application/pdf<br />Departmental Bulletin Paper<br />Polyamines of the planarian, Dugesia tigrina and the leech, Haemadipsa zeylanica were analyzed by high-performance liquid chromatography. An uncommon polyamine, homospermidine,
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in addition to putrescine, cadaverine, spermidine, spermine and agmatine, was detected in the two organisms. Spermidine and spermine levels increased during regeneration in D. tigrina.
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