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TAKAYAMA, Kiyoshige ; KAWATA, Haruka ; TAKAHARA, Ryohta ; TOMITA, Kazuhide
概要:
application/pdf<br />Departmental Bulletin Paper<br />Leptin has been recognized to be an important neuroendocrine signa
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l in the\nregulation of food intake and energy balance. We aimed to survey central neurons that might be\nactivated after peripheral administration of leptin, by examining the distribution of neurons\nexpressing c-Fos protein. Leptin dissolved at a dose of 500 μg/kg in physiological saline was\nintraperitoneally injected in Wistar rats. One and a half hours after the injection, rats were\ntranscardially perfused with saline and fixed with fixatives. The brain was removed and\nsectioned at 40 μm in thickness. Every fourth section was treated with anti-c-Fos antiserum,\nand c-Fos protein was immunohistochemically stained using the avidin-biotin complex method.\nControl rats were injected with saline solution, and brain sections were processed similarly as\ndescribed above. It was found that leptin injected intraperitoneally induced the neuronal\nexpression of c-Fos protein in several nuclei throughout the brain. In the central nucleus\namygdala, ventromedial nucleus of hypothalamus, periaqueductal gray matter, lateral\nparabrachial nucleus, and the solitary tract nucleus, numbers of neurons expressing c-Fos\nprotein were much more in the test experiments than those in the control experiments.\nIntraperitoneally injected leptin was found to stimulate central neurons that may play some\nroles in the regulation of such as a food intake.
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HAYASHI, Kaori ; TAKAYAMA, Kiyoshige
概要:
application/pdf<br />Departmental Bulletin Paper
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Takayama, Kiyoshige ; Iwazaki, Hiromi ; Hirabayashi, Misako ; Ohta, Yuka
概要:
application/pdf<br />Departmental Bulletin Paper<br />The present study was aimed to survey central neurons that might be activated after peripheral administration of apelin-12 (AP12, one of apelin peptide homologues), by
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examining the distribution of neurons expressing c-Fos protein. AP12 has been known to
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