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| 1.
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Hamana, Koei ; Hamana, Hiroshi ; Shinozawa, Takao
概要:
application/pdf<br />Departmental Bulletin Paper<br />Polyamines of the planarian, Dugesia tigrina and the leech, Haemadipsa zeylanica were analyzed by high-performance liquid chromatography. An uncommon polyamine, homospermidine,
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in addition to putrescine, cadaverine, spermidine, spermine and agmatine, was detected in the two organisms. Spermidine and spermine levels increased during regeneration in D. tigrina.
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| 2.
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Hamana, Koei
概要:
application/pdf<br />Departmental Bulletin Paper<br />Cellular polyamines of newly validated seventeen genera belonging to the two taxa of Gram-positive eubacteria were analyzed by HPLC. Brochothrix was devoid of polyamines and
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Globicatella and Dolosignarulum synthesized spermidine within the low G+C subgroup. Appreciable amounts of polyamines were not detected in the high G+C subgroup, Nocardioides, Aeromicrobium and Microlunatus, and the six genera Brachybacterium, Dermcoccus, Kocuria, Kytococcus, Nestenkonia and Luteococcus transferred from the genus Micrococcus. The coryneform Dermbacter produced cadaverine as the major polyamine. The actinomycetes Dermatophilus and Acidothermus, and anaerobic Bifidobacterium and Propionibacterium, belonging to the high G+C subgroup, had no polyamine synthetic ability.
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| 3.
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Hamana, Koei
概要:
application/pdf<br />Departmental Bulletin Paper<br />Polyamines of the nine genera of coryneform and related Gram-positive eubacteria were analyzed by HPLC. Authentic species of Microbacterium, Aureobacterium, Cellulomonas and
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Corynebacterium were devoid of polyamines. Arthrobacter species were divided into polyamine-absent, putrescine, cadaverine and putrescine-cadaverine types. Clavibacter contained putrescine and cadaverine. Spermidine was detected in some species of Brevibacterium, Exiguobacterium and Curtobacterium and diaminopropane in some Curtobacterium. Heterogeneous polyamine profiles were found in the three genera.
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| 4.
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Hamana, Koei
概要:
application/pdf<br />Departmental Bulletin Paper<br />The λ phage DNA and hog thyroid DNA after exposure to a hydroxyl r
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adical-generating system containing NADPH-cytochrome P-450 reductase and anthraquinones such as mitoxantrone (CL 232315) and CL 232468 lacked in intact DNA fragments, indicating DNA strand breaks. The mechanism of DNA scission by the drugs closely resembles that induced by adriamycin and mitomycin C. When isolated hog thyroid chromatin was exposed to the system containing mitoxantrone, fragmentation of chromatin DNA was not detected. DNase I digestion for chromatin DNA was inhibited by the presence of the anthraquinones, suggesting intercalation of the drugs to DNase I-sensitive DNA regions in chromatin without DNA strand breaks.
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| 5.
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Hamana, Koei ; Satake, Sachiko ; Iyobe, Shizuko ; Matsuzaki, Shigeru
概要:
application/pdf<br />Departmental Bulletin Paper<br />Pseudomonas cepacia as well as all five Alcaligenes and three Comamonas species tested belonging to the beta subclass of Proteobacteria ubiquitously contained 2-hydroxyputrescine and
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putrescine as the major polyamines. Cadaverine was found in P. cepacia. Putrescine and spermidine were the major polyamines of Pseudomonas aeruginosa and Pseudomonas putida belonging to the gamma subclass and Pseudomonas saccharophila whose taxonomic positions have not been established. Polyamine distribution profiles can serve as a chemotaxonomic marker within Pseudomonas species. Relative distribution patterns of the major polyamines of P. aeruginosa did not changed at different culture periods in spite of increase or decrease of their levels during growth.
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| 6.
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Hamana, Koei ; Suzuki, Mitsuo
概要:
application/pdf<br />Departmental Bulletin Paper<br />^3H-Estradiol-17β was predominantly distributed in the cytosol and
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nuclear fractions when the steroid hormone was incorporated into liver cells of mature female rainbow trout in vitro. ^3H-Estradiol-bound 4s receptor complex (K_a=1.3×10^8 M^<-1>) was detected in the liver cytosol. A little seasonal variation and sex difference on the estradiol binding were observed in the cytosol receptor fraction of trout liver at various spawing seasons. The female specific and seasonal variable nuclear bindings were found only by the incubation of the isolated nuclei or chromatin with free ^3H-estradiol. The nuclear binding determined in the mature female trout liver shows high binding affinity (K_a=5.7×10^7 M^<-1>) and high binding site (7 pmol/mg protein). ^3H-Estradiol-receptor complex faild to bind estradiol to the isolated nuclei and chromatin. It is suggested that the saturable nuclear binding of free estradiol is proportional to the quantity of the receptors translocated from the cytoplasm. Treatment with 0.3M NaCl, 0.1% heparin, phospholipase or DNase faild to release the bound ^3H-estradiol from the nuclei. A 4s molecule bound with ^3H-estradiol was released from the chromatin acceptor sites by protease digestions. The binding process of estrogen into the nuclei in the trout liver is similar to the transport mechanism found in other oviparous and mammalian livers.
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| 7.
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Hamana, Koei
概要:
application/pdf<br />Departmental Bulletin Paper<br />Electrophoresis in polyacrylamide gels in the presence of 6mM Triton X-100 in 0.9M acetic acid and 6.25M urea gave a high resolution of separation of the histones in which
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methionines and cysteines, respectively, are oxidized to the sulfoxides or sulfones (H2A, H2B, H3, H4) and to form an intramolecular disulfide bond (H3). The methionine-oxidized histones migrated faster than untreated histones. The electrophoretic mobility of histone H3 also increased by oxidation of the two cysteines to a cystine. The increase of the mobilities might be explained in terms of the decrease of the helical contents of the histones by the oxidation of methionine and/or cysteine residues.
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| 8.
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Hamana, Koei
概要:
application/pdf<br />Departmental Bulletin Paper<br />The electrophoretic mobility in polyacrylamide gel of calf thymus histones was examined as a function of the degree of cross-linkage (C,%) of the gel in five different
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buffer systems. With increasing the degree of cross-linkage, the mobility once decreased and then increased, irrespective of total gel concentrations (T,%) used (5-15%). The degree of cross-linkage of the gel where the mobilities of the histones became minimum (C_<min>), 7-15%C, was larger than that where the mobilities of other high molecular weight proteins become minimum, 5-7%C. The retardation coefficients (K_R) of the histones obtained from changes of the total gel concentration attained their maximum values in gels which have C_<min>. In gels of C_<min> an approximately linear relationship was obtained between the K_R and the molecular weight of the histones.
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| 9.
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論文
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Hamana, Koei
概要:
application/pdf<br />Departmental Bulletin Paper<br />Histones and protamines behaved as a monomeric form in 0.9M acetic acid. When gel electrophoresis was carried out in 0.9M acetic acid, the mobilities of calf H2A, H2B, H3 and H4
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histones were decreased proportionaly to the helix content of the histones but the mobilities of calf H1 histone and fish protamines were not decreased by the addition of 6mM nonionic surfactant into the gel. Degree of the decreasing on the mobilities of four histones was also proportional to the molecular weight of the added nonionic surfactant. Structure of hydrophobic group of the surfactants was more affective than polyoxyethylene chain length of the surfactants. H2B, H3 and H4 histones aggregated in 0.05-0.1M sodium phosphate buffer, pH 7.0, while H1 and H2A histones behaved as a monomeric form. Nonionic surfactants bound to H2A histone but did not to H1 histone and to the aggregates of other histones in the buffer.
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| 10.
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論文
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Hamana, Koei
概要:
application/pdf<br />Departmental Bulletin Paper<br />Polyacrylamide gel electrophoresis or chromatography of histories (H1, H5, H2A, H2B, H3 and H4) and protamines (salmine and clupeine) in 0.9M acetic acid or 0.01M HC1,
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respectively, was carried out at various temperatures. A retardation of either electrophoretic migration or chromatographic elution of the proteins was observed at lower temperatures. H1 and H5 histones exhibited the least extent of retardation. The retardation with lowering temperature was also observed in the presence of 0.02-0.05M NaCl but not in the presence of 5-6.25M urea. The extent of retardation in polyacrylamide gel chromatography with lowering temperature increased with proteins of higher arginine contents. The fact was explained as that ionic interactions between guanido groups of arginine residues in the proteins and carboxyl groups of polyacrylamide are strengthened at lower gel temperatures.
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