Effect of Temperature on Polyacrylamide Gel Electrophoresis and Chromatography of Histones and Protamines

フォーマット:

論文

責任表示:

Hamana, Koei

言語:

英語

出版情報:

群馬大学医療技術短期大学部, 1981-03-10

著者名:

Hamana, Koei  

掲載情報:

群馬大学医療技術短期大学部紀要

ISSN:

0389-7540    

巻:

1

開始ページ:

106

終了ページ:

119

バージョン:

VoR

概要:

application/pdf<br />Departmental Bulletin Paper<br />Polyacrylamide gel electrophoresis or chromatography of histories (H1, H5, H2A, H2B, H3 and H4) and protamines (salmine and clupeine) in 0.9M acetic acid or 0.01M HC1, respectively, was carried out at various temperatures. A retardation of either electrophoretic migration or chromatographic elution of the proteins was observed at lower temperatures. H1 and H5 histones exhibited the least extent of … retardation. The retardation with lowering temperature was also observed in the presence of 0.02-0.05M NaCl but not in the presence of 5-6.25M urea. The extent of retardation in polyacrylamide gel chromatography with lowering temperature increased with proteins of higher arginine contents. The fact was explained as that ionic interactions between guanido groups of arginine residues in the proteins and carboxyl groups of polyacrylamide are strengthened at lower gel temperatures. 続きを見る