ヒト脳におけるメタロチオネインの局在について

Format:

Article

Responsibility:

鈴木, 慶二 ; 河原田, ウメ子 ; 中島, 克行 ; 槙田, 洋 ; 小滝, 規子 ; 木村, 正己 ; 田村, 勇次 ; 上原, 克昌

Language:

Japanese

Published:

群馬大学医療技術短期大学部, 1992-03-31

Authors:

鈴木, 慶二

河原田, ウメ子

中島, 克行

槙田, 洋

小滝, 規子

木村, 正己

田村, 勇次

上原, 克昌

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Publication info:

群馬大学医療技術短期大学部紀要

ISSN:

0389-7540    

Volume:

12

Start page:

29

End page:

35

Version:

VoR

Abstract:

application/pdf<br />Departmental Bulletin Paper<br />The localization of metallothionein (MT), a small molecular weight heavy metal binding protein in the human brain, was investigated by the immunohistochemical technique. The amounts of MT and heavy metals (Zn, Cu, Cd) were assayed by radioimmunoassay and atomic absorption spectrophotometer, respectively. Immunohistochemically, MT was found in the pia mater, ependymal cells, … astrocytes and neuropil of the telencephalon. In contrast, oligodendroglia and microglia did not show positive immunostaining for MT. The cytoplasm, processes and some nuclei of astrocytes showed strong MT immunostaining. Vascular feet and adventitia were also postitive for MT immunostaining. Moreover, the pia mater, astrocytes, neuropil and ependyma in the diencephalon, mesencephalon, pons, medulla oblongata and spinal cord showed the same positive immunostaining for MT as the telencephalon. In the cerebellum, Bergmann's glias and astrocytes of the granular layer and white matter showed strongly positivity for the MT immunostain. According to the radioimmunoassay, the amount of MT in the brain was relatively high about 36.0μg/g wet weight. For the heavy metals in the brain, zinc, copper and cadmium were detected. Read more