1.

学位論文(AKAGI収録)

学位
Olia, Alex ; オリア, アレックス
出版情報: 2020-03-24.  群馬大学医学系研究科
概要: 学位記番号:医博甲1797
2.

論文(AKAGI収録)

論文(AKAGI収録)
Kaneko, Yuka ; Kaneko, Yoriaki ; Ohnishi, Hiroshi ; Tomizawa, Takeshi ; Okajo, Jun ; Saito, Yasuyuki ; Okuzawa, Chie ; Murata, Yoji ; Okazawa, Hideki ; Nojima, Yoshihisa ; Okamoto, Koichi ; Matozaki, Takashi
出版情報: The Kitakanto medical journal.  58  pp.133-139,  2008-05-01.  北関東医学会
概要: Background & Aims: SHPS-1 is a transmembrane protein that binds the protein tyrosine phosphatases SHP-1 and SHP-2 throu gh its cytoplasmic region. It is highly expressed on the surface of CD11c+ dendritic cells (DCs) and macrophages. We have recently shown that priming of CD4+T cells by DCs is markedly impaired in mice that express a mutant form of SHPS-1 lacking most of the cytoplasmic region. We have now evaluated further the functions of CD4+T cells derived from SHPS-1 mutant mice. Methods: The expression of cell surface molecules on CD4+T cells was examined by flow cytometry. The proliferation of CD4+T cells was measured by[3H]thymidine incorporation. Cytokine production by CD4+T cells was measured by ELISA. Results: SHPS-1 is expressed at low level on CD4+T cells of wild-type mice. The T cell receptor (TCR)-stimulated proliferation of CD4+T cells from SHPS-1 mutant mice was markedly decreased, whereas the TCR-stimulated production of IL-2 and IFN-γ by these cells was markedly increased, compared with those apparent with wild-type cells. Differentiation of CD4+T cells from SHPS-1 mutant mice into Th1 cells was also impaired. Conclusions: Present results suggest that SHPS-1 is essential for proper regulation of CD4+T cell functions. 続きを見る