※一部利用できない機能があります
| 1.
論文 |
論文
|
Kaneko, Yuka ; Kaneko, Yoriaki ; Ohnishi, Hiroshi ; Tomizawa, Takeshi ; Okajo, Jun ; Saito, Yasuyuki ; Okuzawa, Chie ; Murata, Yoji ; Okazawa, Hideki ; Nojima, Yoshihisa ; Okamoto, Koichi ; Matozaki, Takashi
概要:
application/pdf<br />Journal Article<br />Background & Aims: SHPS-1 is a transmembrane protein that binds the protein ty
…
rosine phosphatases \nSHP-1 and SHP-2 through its cytoplasmic region. It is highly expressed on the surface of CD11c+\ndendritic cells (DCs) and macrophages. We have recently shown that priming of CD4+T cells by DCs \nis markedly impaired in mice that express a mutant form of SHPS-1 lacking most of the cytoplasmic \nregion. We have now evaluated further the functions of CD4+T cells derived from SHPS-1 mutant mice. \nMethods: The expression of cell surface molecules on CD4+T cells was examined by flow cytometry. \nThe proliferation of CD4+T cells was measured by［3H］thymidine incorporation. Cytokine production \nby CD4+T cells was measured by ELISA. Results: SHPS-1 is expressed at low level on CD4+T \ncells of wild-type mice. The T cell receptor (TCR)-stimulated proliferation of CD4+T cells from \nSHPS-1 mutant mice was markedly decreased, whereas the TCR-stimulated production of IL-2 and \nIFN-γ by these cells was markedly increased, compared with those apparent with wild-type cells. \nDifferentiation of CD4+T cells from SHPS-1 mutant mice into Th1 cells was also impaired. Conclusions: Present results suggest that SHPS-1 is essential for proper regulation of CD4+T cell functions.
続きを見る
|
||
| 2.
論文 |
論文
|
Hayashi, Junichi ; Hiromura, Keiju ; Kitahara, Tokuyuki ; Kaneko, Yoriaki ; Kuroiwa, Takashi ; Ueki, Kazue ; Nojima, Yoshihisa
概要:
application/pdf<br />Journal Article<br />Background and Aims : Although serum or plasma thrombomodulin (TM) was reporte
…
d to be increased in various diseases with vascular injuries, blood TM was also shown to be influenced by renal function. In this study, we determined whether serum TM (sTM) could be a marker of vascular injury in patients with myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA)-associated vasculitis, in which kidneys are often affected. Methods : Thirteen patients with MPO-ANCA-associated vasculitis were investigated and 43 patients with chronic glomerulonephritis (CGN) served as a control. sTM was measured by one-step sandwich enzyme immunoassay method. Results : A significant positive correlation was found between sTM and serum creatinine (sCr) (sTM=2.24×sCr+2.16, r=0.91, P<0.001) in patients with CGN. In order to correct sTM for renal function, we calculated corrected sTM according to the following formula : corrected sTM (%)=[measured sTM/expected sTM at the measured sCr]×100=[sTM/(2.24×sCr+2.16)]×100. Patients with MPO-ANCA-associated vasculitis tended to have lower levels of corrected sTM before treatment compared to patients with CGN, but not significantly (86.8±23.2% vs. 98.7±18.3%, ANCA vs. CGN, P=0.12). Corrected sTM was increased significantly within 1 month after the beginning of treatment (145.7±46.9%, P=0.008, vs. before treatment in each patient). In remission, corrected sTM returned to the same levels of CGN (104.5±38.6%, P=0.03, vs. within 1 month in each patient). Conclusions : These results showed that sTM corrected for renal function does not simply reflect disease activity in MPO-ANCA-associated vasculitis.
続きを見る
|
