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Hosoya, Ryuichi ; Hamana, Koei
概要:
application/pdf<br />Departmental Bulletin Paper<br />The present study was aimed to survey central neurons that might b
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e activated after peripheral administration of ghrelin, by examining the distribution of neurons expressing c-Fos protein. In the test experiment, ghrelin (20 , μg/kg) was intravenously inj
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| 2.
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Hosoya, Ryuichi ; Hamana, Koei
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application/pdf<br />Departmental Bulletin Paper<br />The possibility of the absence of cellular triamines in the four flavobacteria, Flammeovirga aprica IAM 14298, 'Flexibacter aurantiacus subsp. Copepodarum' IFO 15978, 'Microscilla
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arenaria' IFO 15982 and Saprospira grandis NCIMB 1363, phylogenetically loc
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| 3.
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Tanaka, Takehiko ; Hamana, Koei ; Itoh, Takashi
概要:
application/pdf<br />Departmental Bulletin Paper<br />The polyamine patterns of 19 species of extremely halophilic archaea (archaebacteria) and 14 species of methanogenic archaebacteria were examined. The acid-extracted cellular
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polyamines were analyzed by HPLC. Appreciable amount of agmatine was detected as
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| 4.
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Ichimura, Sachiko ; Nemoi, Mitsuru ; Hamana, Koei
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application/pdf<br />Departmental Bulletin Paper<br />Changes of cellular polyamine levels in HeLa S3 cells after carbon-ion beam irradiation were determined. The increase in DNA level of the irradiated cells was similar to
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that of nonirradiated population until 40 hr. Putrescine in irradiated cells remains lower level in spite of the remarkable increase in normal cells.
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| 5.
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Hamana, Koei
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application/pdf<br />Departmental Bulletin Paper<br />Polyamines were analyzed for a chemotaxonomic interest in seventeen thiobacilli distributed within the alpha, beta and gamma subclasses of the class Proteobacteria. Thiobacillus
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aquaesulis, T. denitrificans and T. thioparus and three Thiomonas spcies, bel
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| 6.
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Hamana, Koei
概要:
application/pdf<br />Departmental Bulletin Paper<br />Polyamines of 14 eubacteria belonging to the orders Planctomycetales and Verrucomicrobiales and the phyla Fibrobacter and Acidobacterium were analyzed for chemotaxonomic
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evaluation. Planctomyces brasiliensis, P. maris, Pirellula maris, P. staleyi and Isos
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| 7.
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Hamana, Koei ; Hamana, Hiroshi ; Shinozawa, Takao
概要:
application/pdf<br />Departmental Bulletin Paper<br />Polyamines of the planarian, Dugesia tigrina and the leech, Haemadipsa zeylanica were analyzed by high-performance liquid chromatography. An uncommon polyamine, homospermidine,
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in addition to putrescine, cadaverine, spermidine, spermine and agmatine, was detected in the two organisms. Spermidine and spermine levels increased during regeneration in D. tigrina.
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| 8.
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Hamana, Koei
概要:
application/pdf<br />Departmental Bulletin Paper<br />Cellular polyamines of newly validated seventeen genera belonging to the two taxa of Gram-positive eubacteria were analyzed by HPLC. Brochothrix was devoid of polyamines and
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Globicatella and Dolosignarulum synthesized spermidine within the low G+C subgroup. Appreciable amounts of polyamines were not detected in the high G+C subgroup, Nocardioides, Aeromicrobium and Microlunatus, and the six genera Brachybacterium, Dermcoccus, Kocuria, Kytococcus, Nestenkonia and Luteococcus transferred from the genus Micrococcus. The coryneform Dermbacter produced cadaverine as the major polyamine. The actinomycetes Dermatophilus and Acidothermus, and anaerobic Bifidobacterium and Propionibacterium, belonging to the high G+C subgroup, had no polyamine synthetic ability.
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| 9.
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Hamana, Koei
概要:
application/pdf<br />Departmental Bulletin Paper<br />Polyamines of the nine genera of coryneform and related Gram-positive eubacteria were analyzed by HPLC. Authentic species of Microbacterium, Aureobacterium, Cellulomonas and
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Corynebacterium were devoid of polyamines. Arthrobacter species were divided into polyamine-absent, putrescine, cadaverine and putrescine-cadaverine types. Clavibacter contained putrescine and cadaverine. Spermidine was detected in some species of Brevibacterium, Exiguobacterium and Curtobacterium and diaminopropane in some Curtobacterium. Heterogeneous polyamine profiles were found in the three genera.
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| 10.
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Hamana, Koei
概要:
application/pdf<br />Departmental Bulletin Paper<br />The λ phage DNA and hog thyroid DNA after exposure to a hydroxyl r
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adical-generating system containing NADPH-cytochrome P-450 reductase and anthraquinones such as mitoxantrone (CL 232315) and CL 232468 lacked in intact DNA fragments, indicating DNA strand breaks. The mechanism of DNA scission by the drugs closely resembles that induced by adriamycin and mitomycin C. When isolated hog thyroid chromatin was exposed to the system containing mitoxantrone, fragmentation of chromatin DNA was not detected. DNase I digestion for chromatin DNA was inhibited by the presence of the anthraquinones, suggesting intercalation of the drugs to DNase I-sensitive DNA regions in chromatin without DNA strand breaks.
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