Gene Expression Profiles of Prostate Cancer Cell Lines, LNCaP, PC-3 and DU-145, Assessed by cDNA Microarray

フォーマット:

論文

責任表示:

Okamura, Keigo ; Koike, Hidekazu ; Matsui, Hiroshi ; Suzuki, Kazuhiro

言語:

英語

出版情報:

北関東医学会, 2008-11-01

著者名:

• Okamura, Keigo
• Koike, Hidekazu
• Matsui, Hiroshi
• Suzuki, Kazuhiro

掲載情報:

The Kitakanto medical journal = 北関東医学

ISSN:

1343-2826    

巻:

58

通号:

4

開始ページ:

363

終了ページ:

369

バージョン:

VoR

概要:

application/pdf<br />Journal Article<br />Purpose: In the basic research of prostate cancer, prostate cancer cell lines are very valuable tools to\ncharacterize the biology of prostate cancer. Three cell lines including LNCaP, PC-3 and DU-145 are\nfrequently utilized. Characterization of molecular characteristics of them is very important. In the\npresent study, we screened gene expression patterns of human prostate cancer cell lines, LNCaP, PC-3 … and\nDU-145, using cDNA microarray. Methods: Total RNA from LNCaP, PC-3 or DU-145 cells was\nreversely transcribed in the presence of Cy3 or Cy5 as a probe. Mixed probes were hybridized to a slide\nglass carrying 557 kinds of cancer-related known genes. Signal intensities of Cy-3 and Cy-5 were\nseparately scanned, and relative signal ratios of Cy3/Cy5 were analyzed. Gene expression levels of\nseveral genes were quantified by real-time PCR. Results: We detected over-expressed or underexpressed\ngenes in each cell line. Among these genes,farnesyldiphosphate farnesyltransferase 1, amphiregulin,\nE-cadherin, urokinase-type plasminogen activator (uPA), and glutathione peroxidase 1 gene\nexpression levels were quantified using quantitative real-time PCR. The relation of the gene expression\npatterns of these genes was consistent with those assessed by real-time PCR. Conclusions: Gene\nexpression profiles of human prostate cancer cells varied among cell lines. These profiles will provide\nuseful information on prostate cancer cell lines in the design of experiments, analysis of experimental\ndata, and so on. 続きを見る